WAY200070

Boiling point: 463.6±35.0 °C(Predicted)

Density: 1.720±0.06 g/cm3(Predicted)

Storage temp.: RT

Solubility: DMSO: ≥20mg/mL

Form: Solid

Color: White to off-white

1.Cell Experiment

Solubility in DMSO: ≥ 31 mg/mL (101.27 mM; Hygroscopic DMSO significantly affects the solubility of the product, please use freshly opened DMSO)

* "≥" means soluble, but saturation unknown.

Preparing Stock Solutions:

Please choose a suitable solvent to prepare the stock solution based on the product's solubility in different solvents. Once the solution is prepared, aliquot and store it to avoid product degradation caused by repeated freeze-thaw cycles.

Storage conditions and shelf life of the stock solution: -80°C for 2 years; -20°C for 1 year. When stored at -80°C, use within 2 years; when stored at -20°C, use within 1 year.

2.Animal Experiment

Please choose the appropriate dissolution method based on your experimental animals and administration route.

For the following dissolution schemes, please first prepare a clear stock solution according to the In Vitro method, then add solubilizers in sequence:  

—— To ensure the reliability of the experimental results, the clear stock solution can be appropriately stored based on storage conditions; for in vivo experimental working solutions, it is recommended to prepare them fresh and use them on the same day.  

The percentages shown before each solvent below refer to the volume proportion of that solvent in the final solution you are preparing; if precipitation or crystallization occurs during preparation, solubilization can be assisted by heating and/or ultrasonic treatment.

Protocol 1

Add each solvent in sequence: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 10 mg/mL (32.67 mM); clear solution

This method can produce a clear solution of ≥ 10 mg/mL (saturation unknown).

For example, to prepare 1 mL of working solution, add 100 μL of 100.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD saline solution and mix well.

Dissolve 2 g of SBE-β-CD (sulfobutylether β-cyclodextrin) powder in 10 mL of saline, and dissolve completely to form a clear, transparent solution.

Protocol 2

Please add each solvent in order: 10% DMSO, 90% Corn Oil

Solubility: ≥ 10 mg/mL (32.67 mM); clear solution

This method can produce a clear solution with ≥ 10 mg/mL (saturation unknown) and is suitable for use in animal experiments with a study period of more than half a month.

For 1 mL of working solution, take 100 μL of 100.0 mg/mL clear DMSO stock solution and add it to 900 μL of corn oil, then mix well.